The Fort Caroline National Memorial preserves the site of an attempted French colonial settlement on the banks of the St. Johns River in Jacksonville. Established in 1564 it was very quickly sacked by the Spanish. This site is where René Goulaine de Laudonnière first landed to set up the colony. The original site of Fort Caroline has never been determined, but it is believed to have been located near the present-day memorial. A reconstruction is maintained by the park service.
The memorial is administered by the Timucuan Ecological Preserve which is also a National Park Service unit.